Structural and physicochemical properties of endosperm starches possessing different alleles at the amylose-extender and waxy locus in maize (Zea mays L.)
1999
Fuwa, H. | Glover, D.V. | Fujita, S. | Sugimoto, Y. | Inouchi, N. | Sugihara, M. | Yoshioka, S. | Yamada, K.
The structure and physicochemical properties of endosperm starches from seven amylose-extender (ae) and two waxy (wx) alleles of maize (Zea mays L) were studied. Starches prepared from mature kernels of six ae mutants, Oh43 inbredline ae (standard ae), ae-RWB 2 and ae-RWB-3, and W23 x L317 hybrid line ae-PP, ae-Bol 561 and ae-emll, were uniquely ae type, as was concluded from B type X-ray diffractograms; high gelatinization temperatures determined by differential scanning calorimetry (DSC); with poor starch-granule digestibility to amylase; high amylose (37-45%) and high intermediate fraction (13-18%) contents; and low ratios (1.0-1.2) of long alpha-1,4-chains to short alpha-1,4-chains of amylopectin determined by gel permeation chromatography (GPC) of isoamylase-debranched starches. The results also indicated that different ae alleles had different effects on the amylose content of endosperm starches. Mature kernels of Oh43 ae-RWB-1 mutant showed tarnished and translucent phenotype characteristics of the ae genotype but contained endosperm starch with 21-22% of amylose which was lower than that of the Oh43 normal counterpart.
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