The use of ridascreen verotoxin (ELISA) for research of EHEC strains [enterohaemorragic Escherichia coli - meat - dairy products]
1998
Bersani, C. | Ripamonti, C. | Fiori, M. | Cantoni, C. (Milan Univ. (Italy). Istituto di Ispezione degli Alimenti di Origine Animale)
anglais. Some of the common diagnostic methods of an EHEC infection seem to have disadvantages. To prove the pathogenic factor, a verotoxin detection should be carried out on cultures from faeces. This can occur on a genetic basis with PCR or, immunologically with ELISA: in comparison to PCR, the ELISA technique offers a simpler method which can be carried out in every laboratory. In this study 250 strains of E. coli (isolated from dairy-cows faeces) have been examined and only some of them resulted toxigenic (1.6%)
Afficher plus [+] Moins [-]italien. La ricerca di E. coli enteroemorragici e' oggi facilitata dall'impiego della tecnica immunoenzimatica ELISA. E' stata eseguita una ricerca per i ceppi di E. coli isolati da bovine lattifere: solo 1.6% (4 su 250) sono risultati tossinogeni
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