Determination of the selfing rate in a Hinoki (Chamaecyparis obtusa) seed orchard by using a chloroplast PCR-SSCP marker
2000
Seido, K. | Maeda, H. | Shiraishi, S.
The selfing rate in a Hinoki (Chamaecyparis obtusa) seed orchard, representing the most common coniferous species in Japan, was determined by using a highly specific PCR-SSCP (Polymerase Chain Reaction - Single-Strand ConformationPolymorphism) marker which recognizes the spacer region between the genes trnD and trnY of the chloroplast DNA. One- thousand and three open-pollinated offsprings were analyzed from plus tree clone YKZ5. This clone has a single point mutation in comparison with 32 wild type clones which are also present in the orchard. Among the offsprings tested, the mutant chloroplast haplotype was detected in 23 samples. Based on the paternal inheritance of the chloroplast DNA marker, the mean selfing rate ofclone YKZ5 was determined to 2.3% in good agreement with the theoretical value expected. Data demonstrate that the newly- developed PCR-SSCP marker derived from chloroplast DNA provides a powerful tool for accurate and effective analyzing of gene flow within a Hinoki seed orchard.
Afficher plus [+] Moins [-]Mots clés AGROVOC
Informations bibliographiques
Cette notice bibliographique a été fournie par ZB MED Nutrition. Environment. Agriculture
Découvrez la collection de ce fournisseur de données dans AGRIS