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Agrobacterium tumefaciens-mediated transformation and regeneraqtion of carnation (Dianthus caryophyllus L.)

2001

Rafiqi, M.

The work described in this thesis comprises the recovering of transgenic carnation (Dianthus caryophyllus L.) plantlets on Kanamycin-containing medium after co-cultivation of leaf explants with armed strain of Agrobacterium tumefaciens containing a vector pBINPLUS carrying the neomycin phosphotransferase II (NPTII) and Ö-glucuronidase (GUS) genes. Transgenic procedure andpatterns of GUS expression in transgenic plants are described. Additional experiments were carried out to investigate factors affecting the regeneration of transgenic shoots. The GUS activity was easily determined histochemically on the regenerated shoots of different age

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Mots clés AGROVOC

agrobacterium tumefaciens dianthus caryophyllus explant explantes explants genetic engineering kanamicina kanamycin kanamycine plant production regeneration transgenic plants

Informations bibliographiques

Pagination

viii, 72 p.

D'autres materias

Ingenieria genetica; Produccion vegetal; Production vegetale; Plante transgenique; Plantas transgenicas; Genie genetique; Regeneracion

Langue

anglais

Note

Summary (En)

4 tables. 16 fig. 110 ref.

Type

Summary; Thesis; Non-Conventional; Bibliography

Auteur institutionnelle

Centre International de Hautes Etudes Agronomiques Méditerranéennes, Chania (Greece). Institut Agronomique Méditerranéen

Sur AGRIS depuis: 2003-08-15

Format: AGRIS AP

Fournisseur de données

Cette notice bibliographique a été fournie par International Center for Advanced Mediterranean Agronomic Studies - Mediterranean Agronomic Institute of Montpellier

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