Occurrence of different Plum Pox Virus strains in several stone fruit species in Austria
2003
Laimer, M. (University of Natural Resources and Applied Life Sciences, Vienna (Austria). Institute of Applied Microbiology. Plant Biotechnology Unit)Mendonça, D. | Arthofer, W. | Hanzer, V. | Myrta, A. | Boscia, D.
A survey was carried out in Austria to type PPV isolates in stone fruit orchards and hedges along their borders. A total of 181 isolates were typed by serological and molecular (IC-RT-PCR and PCR/RFLP in the CP and the Nib-genes) techniques. Selected isolates (54) were tested by DASI-ELISA using the following monoclonal antibodies (MAbs): 5B (universal), 4DG5 (PPV-D specific), AL (PPV-M specific), AC (PPV-C specific) and EA24 (PPV-El Amar specific). The vast majority of Austrian PPV isolates (159) belong to D strain and only a few isolates (16) could be assigned for sure to PPV-M. For 6 isolates, the serological and molecular data for strain identification were not in agreement, which could be explained by sequencing analyses.
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