[Detection and characterisation of Grapevine leafroll associated virus-4 and 5 (GLRaV-4 and GLRaV-5)] | Détection ed caractérisation des virus 4 et 5 associés à l'enroulement foliaire de la vigne (GLRaV-4 et GLRaV-5)
2002
Dridi, M. A.
Two polyclonal antisera were produced against a Mediterranean GLRaV-4 isolate and a GLRaV-5 isolate.A RT-PCR protocol was standardised for the two viruses.Two diagnostic methods i.e. ELISA and RT-PCR were comparatively applied for their validation and to provide information about the dissemination of both viruses.The antiserum developed against GLRa V-5 may be used for immune electron microscopy, whereas it is not reliable for routine application in ELISA.When the antiserum produced to GLRa V-4 was compared with an American antiserum to the type strain of the same virus, the two antisera reacted in a different and unexpected manner: The isolate LR106 was not recognised by As-LR4/BA and KOUDSI Y252 was not identified by the American antiserum; moreover, the analysis of a higher number of samples confirmed the different reactivity of the two antisera.IEM partially confirmed ELISA results.
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