Annotating genomes with massive-scale RNA sequencing
2008
Denoeud, France | Aury, Jean-Marc | Da Silva, Corinne | Noel, Benjamin | Rogier, Odile | Delledonne, Massimo | Morgante, Michele | Valle, Giorgio | Wincker, Patrick | Scarpelli, Claude | Jaillon, Olivier | Artiguenave, François
Next generation technologies enable massive-scale cDNA sequencing (so-called RNA-Seq). Mainly because of the difficulty of aligning short reads on exon-exon junctions, no attempts have been made so far to use RNA-Seq for building gene models de novo, that is, in the absence of a set of known genes and/or splicing events. We present G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq and demonstrate its utility on the grapevine genome.
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