Expression of VP7, a Bluetongue virus group specific antigen by viral vectors: Analysis of the induced immune responses and evaluation of protective potential in sheep
2014
Bouet Cararo, Coraline | Contreras, Vanessa | Caruso, Agathe | Top, Sokunthea | Szelechowski, Marion | Bergeron, Corinne | Viarouge, Cyril | Desprat, Alexandra | Relmy, Anthony | Guibert, Jean-Michel | Dubois, Eric | Thiery, Richard | Bréard, Emmanuel | Bertagnoli, Stéphane | Richardson, Jennifer | Foucras, Gilles | Meyer, Gilles | Schwartz Cornil, Isabelle | Zientara, Stephan | Klonjkowski, Bernard
Bluetongue virus (BTV) is an economically important Orbivirus transmitted by biting midges to domestic and wild ruminants. The need for new vaccines has been highlighted by the occurrence of repeated outbreaks caused by different BTV serotypes since 1998. The major group-reactive antigen of BTV, VP7, is conserved in the 26 serotypes described so far, and its role in the induction of protective immunity has been proposed. Viral-based vectors as antigen delivery systems display considerable promise as veterinary vaccine candidates. In this paper we have evaluated the capacity of the BTV-2 serotype VP7 core protein expressed by either a non-replicative canine adenovirus type 2 (Cav-VP7 R-0) or a leporipoxvirus (SG33-VP7), to induce immune responses in sheep. Humoral responses were elicited against VP7 in almost all animals that received the recombinant vectors. Both Cav-VP7 R-0 and SG33-VP7 stimulated an antigen-specific CD4(+) response and Cav-VP7 R-0 stimulated substantial proliferation of antigen-specific CD8(+) lymphocytes. Encouraged by the results obtained with the Cav-VP7 R-0 vaccine vector, immunized animals were challenged with either the homologous BTV-2 or the heterologous BTV-8 serotype and viral burden in plasma was followed by real-time RT-PCR. The immune responses triggered by Cav-VP7 R-0 were insufficient to afford protective immunity against BTV infection, despite partial protection obtained against homologous challenge. This work underscores the need to further characterize the role of BTV proteins in cross-protective immunity.
Afficher plus [+] Moins [-]Mots clés AGROVOC
Informations bibliographiques
Cette notice bibliographique a été fournie par Fundamental Library of Latvia University of Life Sciences and Technologies
Découvrez la collection de ce fournisseur de données dans AGRIS