Gram-scale production of a basidiomycetous laccase in Aspergillus niger
2014
Zhou, Simeng | Cusano, Angela M | Record, Eric | Lomascolo, Anne | Robert, Viviane | Simaan, A Jalila | Rousselot-Pailley, Pierre | Ullah, Sana | Chaspoul, Florence | Tron, Thierry
We report on the expression in Aspergillus niger of a laccase gene we used to produce variants in Saccharomyces cerevisiae. Grams of recombinant enzyme can be easily obtained. This highlights the potential of combining this generic laccase sequence to the yeast and fungal expression systems for large-scale productions of variants.
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