Prebiotic Activity of Poly- and Oligosaccharides Obtained from Plantago major L. Leaves
2020
Lukova, Paolina | Nikolova, Mariana | Petit, Emmanuel | Elboutachfaiti, Redouan | Vasileva, Tonka | Katsarov, Plamen | Manev, Hristo | Gardarin, Christine | Pierre, Guillaume | Michaud, Philippe | Iliev, Ilia | Delattre, Cédric | Department of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University—Plovdiv, 4002 Plovdiv | Department of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University Paisii Hilendarski, 4000 Plovdiv | Biologie des Plantes et Innovation - UR UPJV 3900 (BIOPI) ; Université de Picardie Jules Verne (UPJV)-Transfrontalière BioEcoAgro - UMR 1158 (BioEcoAgro) ; Université d'Artois (UA)-Université de Liège-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA) ; Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Université d'Artois (UA)-Université de Liège-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA) ; Université catholique de Lille (UCL)-Université catholique de Lille (UCL) | Department of Pharmaceutical sciences, Faculty of Pharmacy, Medical University—Plovdiv, 4002 Plovdiv | Department of Medical Informatics, Biostatistics and e-Learning, Faculty of Public Health, Medical University—Plovdiv, 4002 Plovdiv | Institut Pascal (IP) ; Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne) ; Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA)
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Afficher plus [+] Moins [-]anglais. The aim of the present study was to evaluate the prebiotic potential of Plantago major L. leaves water-extractable polysaccharide (PWPs) and its lower molecular fractions. The structure of PWPs was investigated by high pressure anion exchange chromatography (HPAEC), size exclusion chromatography coupled with multi-angle laser light scattering detector (SEC-MALLS) and Fourier-transform infrared (FTIR) spectroscopy. The chemical composition and monosaccharide analyses showed that galacturonic acid was the main monosaccharide of PWPs followed by glucose, arabinose, galactose, rhamnose and xylose. FTIR study indicated a strong characteristic absorption peak at 1550 cm−1 corresponding to the vibration of COO− group of galacturonic acid. The PWPs was subjected to hydrolysis using commercial enzymes to obtain P. major low molecular fraction (PLM) which was successively separated by size exclusion chromatography on Biogel P2. PWPs and PLM were examined for in vitro prebiotic activity using various assays. Results gave evidence for changes in optical density of the bacteria cells and pH of the growth medium. A heterofermentative process with a lactate/acetate ratio ranged from 1:1 to 1:5 was observed. The ability of PLM to stimulate the production of certain probiotic bacteria glycohydrolases and to be fermented by Lactobacillus sp. strains was successfully proved.
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