Immunoglobin Titer of Suckling Kids as a Result of Colostrum Intake | In Vitro Anti-Proliferation and Anti-Invasion Activities of the Combination Between Recombinant Canine Interferon ( rCaIFN) with Luffa cylindrical Seed Methanol and Chloroform Extracts on MCM-B2 Derived Tumor Cell Line In Collagen Gel Medium | Physiological Status of Indoor Sheep in the Tropical Rain Forest (HPGW) Environtment | Proceeding Of The Mini Workshop East Asia Germany Alumni Network (SEAG)

This study is concerned with the supply of newborn kids with antibodies via colostrums. Twenty kids were assigned to three groups, the first of which received Ccolostrum within the first hour after birth, the second and third after after 4h and 8h, respectively. The first four feedings took place at 4h intervals, the amount fed being 57.5 g/kg body weight lgG1, lgG2, lgA and lgM concentrations were assessed in the blood of the mother, in milk and in the blood of the kids. In each treatment group the serum concentration of all immunoglobulin rose soon after the first uptake of colostrums. It peaked after 24h, waning gradually until the end of the third week. The highest mean lgG1 concentration reached in the group receivinig Colostrum immediately after birt (Group1), the lowest in Group 3 (53.8, 45.3 and 36.6 mg/ml for lgA, whereas the kids of Groups 1 and 2 reached maximum values of 0.3 and 0.7 mg/ml for lgG2 and lgA, respectively. Maximum lgM concentrations were reached 20h p.p. (3.9 mg/ml) in Group 1 and 24h p.p (3.7 mg/ml) in Group 2. They were maintained at that level until 40h p.p.. Kids of Group 3 reached maximum concentrations of 4.2 mg/ml at 32h p.p., subsiding soon thereafter. The concentrations in maternal blood serum of lgG1, lgG2, and lgM showed a slight, statistically non-significant decrease until 12 hours after parturition. The lgA concentrations remained unchanged in the range of 0.2 to 0.3 mg/ml. The concentrations of the four immunoglobulins in maternal milk showed, a distinct decrease immediately after parturition. Within the first 48h they dropped by 90 to 96% and remained at a very low level.

In vitro antiproliferation and anti-invasion activity of the combination between recombinant canine interferon (rCalFN) and Luffa cylindrica seed methanol and chloroform extracts on MCM-B2 tumor cell lines was studies. The dose of the extract was 100 ppm for the chloroform exctract extract and 350 ppm for the methanol extract, while the dose of rCaIFN was 10⁴ IU/ml. The highest anti-proliferation activity of the methanol extract- rCaIFN combination was 84% while the chloroform extract- rCaIFN combination was 60%. The anti-invasion activity was detected on the semi solid medium of collagen gel on both extract combinations even there was a variation on the inhibition capacity among them. The methanol exctract gave the highest inhibition of the cell invasion activity. The ability of the inhibition of cell invasion activity was similar to that of anti_proliferation activity. The result of the present study indicated that the combination of Luffa cylindrica and rCaIFN have a sysynergetic effect on the inhibition of cell invasion and we concluded that this combination give a promising hope for the tumor disorders treatment. The mechanisms of this combination activity is still unclear and under investigation.

As an attempt to improve the animal health and production. Agrosilvopastural (ASP) system has been introduced in the tropical countries. ASP system usually was established as a pasture of animal in the agriculture land or forestry environment. Otherwise development ASP system with indoor animal in the tropical rain forest environtment are still scare. Establishing of the animal production system in the tropical forest environment has a certain consequence related to the influence of physiological status and animal health. The objective of the study is to evaluate the physiological status of indoor sheep in the Gunung Walat Education Forest (HPGW)-IPB, Sukabumi-west Java-Indonesia which has tropical rain forest climatic type. Ten Javanese thin-tailed ewes. Average body weight of 25 kg. In the indoor stable system were feed and water ad libitum under 24 hours continues monitoring of stable humidity and temperature. Measurement of hearth rate, respiration rate, and body temperature were carrying out to the ech ewe in the morning and afternoon. This study reveals that the average humidity in the stable a day in the HPGW-IPB is (97.52 ± 4.87) % rel. and average temperature a day is (22.26 ± 1.62)⁰C. The consequence of bioclimatic condition is directly to the physiological status of the ewes, such a hearth rate by (71.00 ± 10.51). Respiration rate by (29.25 ± 5.39) and body temperature by (38.73 ± 0.56)⁰C. The average humadity a day in the stable shows uncomfortable for the animal health and production system, although the stable temperature is still comfortable enough. The condition is dominantly influenced by density and diversity of vegation in the tropical rain forest. The high humidity in the stable affects enhancing of respiration rate of tehe ewes, although hearth rate and body temperature tend to the normal physiological value.