PRODUCTION OF HORMONE-FREE MONOSEX POPULATION OF NILE TILAPIA, OREOCHROMIS NILOTICUS (LINNAEUS, 1758)
2023
Ravindragouda Patil
The possibility of production of hormone-free all male Nile tilapia (Oreochromis niloticus) in F2 generation, through the production of YY super males in F1 generation, was investigated in the present study. Feminization of Nile tilapia was carried out using the hormone, 17 beta estradiol at 100, 200 and 300 mg/kg concentrations, orally through feed. The hormone enhanced the growth significantly (p<0.05) both in terms of length (55.39±0.67 cm) and weight (55.39±0.67 g) at 300 mg/kg feed when compared to other treatments and control. With the increasing concentrations (300 mg/kg) of the hormone, the survival rate decreased significantly (p<0.05) to 62±1.53%. The feminization rates increased significantly (p<0.05) with increasing concentrations of the hormone, with the highest feminization percentage of 98.27±0.87% observed at 300 mg/kg concentration of the hormone. Two fish from F generation with the PIT tag numbers 81 and 88 produced all male progeny which was confirmed through morphological examination. The genotype of these two YY supermales was re-confirmed through molecular genotyping with the help of primers amhX1+36 and amhdY+5 DNA markers, specific to X and Y chromosomes, respectively using RAPD-PCR.
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