Identification and factors affecting in-vitro growth of an indigenous mushroom, Boletussp. from Bachok, Kelantan, Malaysia
2017
Lau, M. F. | Rosnida T. | Masratul Hawa M. | Latiffah Z.
Wild mushrooms including Boletus are among edible mushrooms consumed by local populations. However, the species identity for many types of wild edible mushroom in Malaysia is poorly known. The present study was conducted to identify and to determine factors affecting in-vitro growth of an edible Boletus sp. (kulat gelam) commonly found in peat swamp forests in Bachok, Kelantan. Identification was initially done using macroscopic and microscopic characteristics of the fruiting bodies. However, due to overlapping chemical colour reactions, morphological and anatomical characteristics of the fruiting body with other Boletus species, species identity was confirmed using ITS region, and the mushroom was identified as Boletus griseipurpureus. A toxicity test indicated that B. griseipurpureus is an edible mushroom with low toxic levels (LC50=4.33 mg/mL). From growth studies, the results suggested that potato dextrose agar (PDA), cassava dextrose agar (CDA) and yeast malt extract (YME) were the most suitable artificial media for mycelial growth of B. griseipurpureus at pH 6.0 and 30°C. To our knowledge, this is the first documented report on wild edible mushroom B. griseipurpureus in Malaysia.
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