Fractionation of berry seeds and pomace using green high-pressure extraction methods
2023
Grabauskaite, Rugile | Juriene, Laura | Kazernaviciute, Rita | Venskutonis, Petras Rimantas
An efficient management of biomass remaining after berry processing gained a lot of attention in the recent years due to their nutritional and other valuable properties. High-value ingredients obtained after biorefining berry pomace are finding application in food, cosmetic and pharmaceutical fields. The aim of this work was to isolate valuable fractions from strawberry, blackberry and elderberry seeds as well as rowanberry pomace by supercritical CO2 (SFE-CO2) and pressurized liquid (PLE) extractions. The highest lipophilic fraction yield by SFE-CO2 was 14.28 ± 0.18% obtained from elderberry seeds. PLE with ethanol and water mixture (70:30) yielded 20.61–47.29% of polar extracts. Tocopherols and phytosterols were determined in lipophilic fractions after Soxhlet extraction. Total phenolic content (TPC) by Folin-Ciocalteu method and antioxidant activity by ABTS•+ scavenging and cupric reducing antioxidant capacity (CUPRAC) assays were investigated of the obtained polar fractions. The highest TPC was determined in strawberry seed PLE extract (92.18 ± 3.90 mg GAE gE−1), while blackberry seed PLE extract exhibited the best radical scavenging and CUPRAC values. TPC, ABTS•+ and CUPRAC values were also evaluated in raw berry seeds and pomace as well as in the residues after each extraction by Quencher methodology. It can be concluded that fractionation of strawberry, blackberry and elderberry seeds and rowanberry pomace by high-pressure extraction enables obtaining the extracts containing valuable bioactive components and possessing antioxidant properties.
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