The functionality of a therapeutic antibody candidate restored by a single mutation from proline to threonine in the variable region
2023
Hautiere, Marie | Maffucci, Irene | Costa, Narciso | Herbet, Amaury | Essono, Sosthene | Padiolleau-Lefevre, Séverine | Boquet, Didier | Service de Pharmacologie et Immunoanalyse (SPI) ; Médicaments et Technologies pour la Santé (MTS) ; Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Génie Enzymatique et Cellulaire (GEC) ; Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS) | Medical Biotechnology Engineering LLC | HPC/AI resources from GENCI-IDRIS [Grant 2022- AD010713707] | ANR-19-CE18-0013,DualmAb,Double marquage (TEP et fluorescence) d'anticorps ciblant les récepteurs aux endothélines(2019)
International audience
Afficher plus [+] Moins [-]anglais. mAbs play an essential role in the therapeutic arsenal. Our laboratory has patented the Rendomab-B49 mAb targeting the endothelin B receptor (ET$_B$). This G protein-coupled receptor plays a driving role in the progression of numerous cancers. We chimerized our mAb (xiRB49) to evaluate its preclinical therapeutic efficacy in different ET$_B$$^+$ tumor models with an antibody drug conjugate approach. As previously reported, the chimerization process of an antibody can alter its functionality. In this article, we present the chimerization of RB49. xiRB49 purified by Protein A remained perfectly soluble and did not aggregate, but it lost all its ability to recognize ET$_B$. A detailed analysis of its variable region using IMGT tools allowed us to identify an unusual proline at position 125. In silico mAb modeling and in vitro experiments were performed for a better understanding of xiRB49 structure-function relationships. Our results show that the proline in position 125 on the heavy chain alters the xiRB49 CDR3 light chain conformation and its mutation to threonine allows complete functional recovery.
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