Assessment of adverse impacts of glyphosate-based herbicide, Excel Mera 71 by integrating multi-level biomarker responses in fishes

Samanta, P.; Pal, S.; Mukherjee, A. K.; Senapati, T.; Jung, J.; Ghosh, A. R.

Assessment of adverse impacts of glyphosate-based herbicide, Excel Mera 71 by integrating multi-level biomarker responses in fishes

2019

This work was aimed to investigate the adverse impacts of Excel Mera 71 on Anabas testudineus, Heteropneustes fossilis and Oreochromis niloticus under laboratory (17.2 mg/L) and field (750 g/acre) conditions based on multiple-level integrated biomarker responses. Haematological parameters were influenced significantly and showed species specificity after Excel Mera 71 exposure under both the conditions. Physiological indices (hepato-somatic index and condition factor) showed higher values; however, hepato-somatic index induced significantly under both the conditions (p < 0.05). Total nuclear abnormalities at molecular level revealed enhanced level. Responses were more adverse in laboratory (O. niloticus > H. fossilis > A. testudineus), while total nuclear abnormalities under field showed the order as: H. fossilis (0.49 ± 0.08) > A. testudineus (0.46 ± 0.10) > O. niloticus (0.27 ± 0.05). Moreover, multi-level integrated biomarker responses displayed responses as: O. niloticus (6.97 fold) > H. fossilis (4.01 fold) > A. testudineus (2.33 fold) in laboratory; however, under field condition, responses were totally different [A. testudineus–H. fossilis (3.98 fold) > O. niloticus (3.01 fold)]. Additionally, glyphosate concentration was significantly correlated with several multi-level biomarker responses (p < 0.05). Moreover, multi-level integrated biomarker responses clearly indicated the higher impacts under laboratory experiment. Present investigation documented that multi-level integrative biomarker responses should be considered as reliable tool for assessment of herbicidal intoxication.

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