Quantitative analysis of γ–oryzanol content in cold pressed rice bran oil by TLC–image analysis method
2014
Sakunpak, Apirak | Jirapornchai Suksaeree, | Monton, Chaowalit | Pathompak, Pathamaporn | Kritsanā Kraisin,
To develop and validate an image analysis method for quantitative analysis of γ-oryzanol in cold pressed rice bran oil.TLC-densitometric and TLC-image analysis methods were developed, validated, and used for quantitative analysis of γ-oryzanol in cold pressed rice bran oil. The results obtained by these two different quantification methods were compared by paired t-test.Both assays provided good linearity, accuracy, reproducibility and selectivity for determination of γ-oryzanol.The TLC-densitometric and TLC-image analysis methods provided a similar reproducibility, accuracy and selectivity for the quantitative determination of γ-oryzanol in cold pressed rice bran oil. A statistical comparison of the quantitative determinations of γ-oryzanol in samples did not show any statistically significant difference between TLC-densitometric and TLC-image analysis methods. As both methods were found to be equal, they therefore can be used for the determination of γ-oryzanol in cold pressed rice bran oil.
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