Development of the chromatographic fingerprint of Scutellaria barbata D. Don by GC–MS combined with Chemometrics methods
2011
Pan, Ruijing | Guo, Fangqiu | Lü, Hongmei | Feng, Wei-wei | liang, Yi-zeng
Gas chromatography fingerprint of Scutellaria barbata D. Don (SB) from different origins was studied by gas chromatography–mass spectrometry (GC–MS) and related Chemometrics methods. The constituents of essential oil of Scutellaria barbata D. Don (SB) and its two adulterants Oldenlandia diffusa (OD) and Lobelia chinensis Lour (LCL) were analyzed and compared, 50, 36 and 38 components were identified from SB, OD and LCL, respectively, there were 16 and 18 common components between SB and OD, SB and LCL. Nine different samples collected from different producing areas of SB were studied. The relative standard deviations (RSDs) of retention time and peak area of each component were less than 0.2% and 6%, respectively. The number of common peaks was up to 52 and the un-common peak area was less than 10%. The similarity and difference among SB and its adulterants were also evaluated by correlation coefficient similarity analysis and principal component analysis, the result showed that developed fingerprint characterize the SB from different producing areas and it is useful and feasible for the discrimination of its adulterants.
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