Comparison of kernel wax from corn genotypes resistant or susceptible to Aspergillus flavus
1997
Russin, J.S. | Guo, B.Z. | Tubajika, K.M. | Brown, R.L. | Cleveland, T.E. | Widstrom, N.W.
Kernels of corn genotype GT-MAS:gk are resistant to Aspergillus flavus. Earlier studies showed that this resistance is due in part to kernel pericarp wax. Experiments were conducted to compare wax from GT-MAS: gk kernels with that from kernels of several susceptible commercial hybrids. GT-MAS:gk had more pericarp wax than did the susceptible hybrids. Scanning electron microscopy revealed that GT-MAS:gk kernels appeared rough and showed abundant wax deposits on kernel surfaces. Susceptible kernels appeared much more smooth and lacked the abundant surface deposits observed in GT-MAS:gk. In vitro bioassays showed that kernel wax from GT-MAS :gk reduced A flavus colony diameter by 35%. Colony diameters on a medium amended with wax from susceptible kernels did not differ from those of controls. Thin-layer chromatography and analyses of chromatograms using NIH Image software showed a distinctive composition for GT-MAS:gk kernel wax. Chromatograms of wax from GT-MAS:gk contained a peak unique to this genotype, but also lacked a peak common to all susceptible hybrids. This is the first report of specific kernel factors involved in resistance to A. flavus in corn.
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