Collaborative study of three screening methods for aflatoxin in corn
1973
Shotwell, O.L. | Stubblefield, R.D.
The 3 rapid screening methods studied all involved chromatography of partially purified extracts on minicolumns. In one method acetonitrile-water is used to extract aflatoxin from corn and a lead acetate precipitation removes impurities from the extracts. The second method specifies acetone-water as the extracting solvent and ammonium sulphate as the precipitant. The third method is based on acetone-water as the extracting solvent and ferric gel as the precipitant. The most sensitive method was the ferric gel precipitation, which could detect 5 parts/billion aflatoxin, but it was very time-consuming. The most satisfactory method (timewise) used the ammonium sulphate and had a sensitivity of 10 parts/billion. This method also led to the best differentiation between levels of aflatoxin. These 2 methods have been adopted as official first action.
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