Biodegradation of chlorimuron-ethyl and the associated degradation pathway by Rhodococcus sp. D310-1

Li, Chunyan; Zang, Hailian; Yu, Qi; Lv, Tongyang; Cheng, Yi; Cheng, Xiaosong; Liu, Keran; Liu, Wanjun; Xu, Pianpian; Lan, Chuanzeng

Biodegradation of chlorimuron-ethyl and the associated degradation pathway by Rhodococcus sp. D310-1

2016

Chlorimuron-ethyl is a typical long-term residual sulfonylurea herbicide, and strategies for its removal have attracted increasing attention. Microbial degradation is considered the most acceptable dissipation method. In this study, we optimized the cultivation conditions (substrate concentration, pH, inoculum concentration, and temperature) of the chlorimuron-ethyl-degrading bacterium Rhodococcus sp. D310-1 using response surface methodology (RSM) to improve the biodegradation efficiency. A maximum biodegradation rate of 88.95 % was obtained. The Andrews model was used to describe the changes in the specific degradation rate as the substrate concentration increased. Chlorimuron-ethyl could be transformed with a maximum specific degradation rate (q ₘₐₓ), half-saturation constant (K S), and inhibition constant (K ᵢ) of 0.4327 day⁻¹, 63.50045 mg L⁻¹, and 156.76666 mg L⁻¹, respectively. Eight biodegradation products (2-amino-4-chloro-6-methoxypyrimidine, ethyl 2-sulfamoyl benzoate, 2-sulfamoyl benzoic acid, o-benzoic sulfimide, 2-[[(4-chloro-6-methoxy-2-pyrimidinyl) carbamoyl] sulfamoyl] benzoic acid, ethyl 2-carbonyl sulfamoyl benzoate, ethyl 2-benzenesulfonyl isocyanate benzoate, and N,N-2(ethyl formate)benzene sulfonylurea) were identified, and three possible degradation pathways were proposed based on the results of high performance liquid chromatography HPLC, liquid chromatography tandem mass spectroscopy (LC-MS/MS), and Fourier transform infrared spectroscopy (FTIR) analyses and the relevant literature. This systematic study is the first to examine the chlorimuron-ethyl degradation pathways of the genus Rhodococcus.

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