Development of 12 new SSR markers for genetic diversity and structure analysis in pistachio (Pistacia vera L.)
2014
Kolahi-Zonoozi, SH. | Mardi, M. | Zeinalabedini, M. | Pirseyedi, S. M. | Mahmoodi, P. | Tabatabaei, I. | Mosavi-Derazmahalleh, S. M. | Farsi, M. | Ebrahimi, M. A. | Ahmadi, K.
Pistachio (Pistacia vera L.) is an important horticultural crop with valuable nuts. So far, only a limited number of microsatellite (simple sequence repeat; SSR) markers have been developed for members of the genus Pistacia, among which, only a few have shown polymorphism among genotypes of Iranian pistachio. This limits precise genotyping and diversity studies on this commercially valuable species. In the present study, we report the development of 12 novel microsatellite (SSR) markers isolated from a repeat sequence-enriched genomic library of P. vera L. We assessed genetic structure and diversity among 45 commercially important Iranian cultivars using these 12 markers. Polymorphic information contents (PIC) varied from 0.19 – 0.56, with an average of 0.33. The mean values of the observed (Hₒ) and expected (Hₑ) heterozygosities were 0.490 and 0.345, respectively. A Neighbor-Net analysis divided our 45 cultivars in two main groups.The results of STRUCTURE software indicated the admixed genetics background of our samples. These 12 new (SSR) markers may prove to be useful for future breeding programmes, and for genetic structure and diversity studies, and facilitate gene mapping analysis and germplasm management.
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