Establishment of CTAB Turbidimetric method to determine hyaluronic acid content in fermentation broth
2009
Chen, Yong-Hao | Wang, Qiang
The characters of visible spectra on the formation of turbidity between hyaluronic acid (HA) and cetyltrimethylammonium bromide (CTAB) were investigated. The effects of reaction time and temperature on the formation of turbidity were also explored. Consequently, CTAB Turbidimetric method (CTM), a quick and safe method to determine HA content in large batch fermentation broth samples, was established. CTM and Bitter–Muir method (BMM) were respectively applied to measure HA content and were thoroughly compared. The results indicate that CTM was superior to BMM in terms of accuracy, precision and sensitivity. CTM is a promising alternative to BMM to determine HA content in fermentation broth.
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