Preliminary investigations on somatic embryogenesis from leaf discs of red oak (Quercus rubra L.)
1996
Rancillac, M. | Klinguer, A. | Klinguer, S. | Millet, B.
Somatic embryos were obtained from leaf discs of juvenile red oak plants. Basal inductive nutrient medium was a modified Murashige and Skoog solution enriched with 500 mg L-1 casein hydrolysate, 100 mg L-1 polyvinylpyrrolidone, 5.4 micromolar naphthaleneacetic acid and 0.09 micromolar benzyladenine. Embryogenesis was obtained only from leaf discs in the presence of light and increased when the adaxial surface of the explants (with midrib or main veins present) was in contact with the medium. Large variation was observed in all experiments. Recurrent embryogenesis was observed at the base of embryo clusters with callus present; conversely, embryogenic potential was rapidly lost by subculturing full call). Maturation, germination and development of isolated somatic embryos were obtained. However, the vast majority of embryos did not have viable apical bud meristems and on only a few occasions were shoots produced.
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