Structural elucidation and physiological properties of genuine soybean saponins
1994
Kudou, S. | Tonomura, M. | Tsukamoto, C. | Uchida, T. | Yoshikoshi, M. | Okubo, K.
The composition and the structures of group B saponins in native soybean seeds were investigated. Five kinds of saponins named soyasaponins alpha g, beta g, beta a, gamma g and gamma a according to elusion order from HPLC were isolated, and the structures were characterized as having a 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP) group attached by acetal linkage to C-22 of the aglycones soyasaponin V, I, II, III and IV respectively. These DDMP conjugated saponin are considered to be the genuine saponins in native soybean seeds, and soyasaponins I, II, III, IV and V are artifacts produced by heating during the extraction or concentration process. Maltol produced by amino-carbonyl reaction was identified after heating an aqueous solution of DDMP conjugated saponins. In addition, soyasaponin beta g is same as the phytochrome killer chromosaponin I. DDMP conjugated saponins possess several biological activities.
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