17β-estradiol regulates prostaglandin E2 and F2α synthesis and function in endometrial explants of cattle
2020
Li, Qianru | Zhang, Shuangyi | Mao, Wei | Fu, Changqi | Shen, Yuan | Wang, Yijing | Liu, Bo | Cao, Jinshan
Prostaglandins (PG) have primary functions in the reproductive tract, however, the mechanism of regulation of PG secretion in the endometrium is unclear. Estrogen as a predominant regulator of uterine functions during the mammalian estrous cycle and effects of estrogen on synthesis of PG and function in uterine tissues of cattle are not fully understood. In this study, there was evaluation of the concentration- and time-effects of 17β-estradiol on PG synthesis in endometrial explants of cattle, focusing on the secretion of prostaglandin E₂ (PGE₂) and prostaglandin F₂α (PGF₂α) as well as relative abundance of mRNA transcript and protein for both the enzymes responsible for PGE₂ and PGF₂α synthesis, including prostaglandin-endoperoxide synthase 1 and 2 (PTGS1, PTGS2), PGE₂ synthase (PGES), PGF₂α synthase (PGFS), and carbonyl reductase (CBR1), and the receptors responsible for downstream PGE₂ (PTGER2, PTGER4) and PGF₂α (PTGFR) signaling. Results indicated that 17β-estradiol increased PGE₂ and PGF₂α production at concentrations ranging from 10⁻¹¹ to 10⁻⁸ M. Furthermore, abundances of PTGS1, PTGS2, PGES, PGFS, PTGER2, PTGER4, and PTGFR mRNA transcripts and protein were greater immediately after 17β-estradiol treatment at almost all the concentrations, while these CBR1 abundances were less as a result of treatments with 17β-estradiol. These data support the hypothesis that estradiol modulates the synthesis and function of PG in the endometrium of cattle.
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