Acetylacylglycerol formation by lipase in microaqueous milieu: effects of acetyl group donor and environmental factors
1995
Kuo, S.J. | Parkin, K.L.
Of six lipases evaluated, lipozyme was most efficacious at mediating acyl-exchange reactions in forming acetylacylglycerols (AcAG) from acetyl donors and olive oil triacylglycerols (TAG) in the absence of organic solvent. Reactivity among a series of 10 acetyl-donor substrates was compared for model reactions configured for acetyl transfer to 1-undecanol and ac(et)yl exchange with olive oil. Of the two acetyl donors most suitable for exchange reactions with olive oil, triacetin was a more efficient cosubstrate than was ethyl acetate. Greatest absolute yields of total AcAG were observed at molar ratios of triacetin/olive oil TAG of 1:2 and ethyl acetate/olive oil TAG of about 3:1, and respective reaction yields approached 90% and 50% of the calculated maximum for these two systems. AcAG formation was optimal at about 1-2% total water content in the system and at 80-85 degrees C. When organic solvents were used as an inert dispersing medium, those of intermediate polarity were most supportive of biocatalysis.
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