Benzothiazole-Based Fluorescent Sensor for Ratiometric Detection of Zn(II) Ions and Secondary Sensing PPi and Its Applications for Biological Imaging and PPase Catalysis Assays
2017
Chang, Chengcheng | Wang, Fang | Wei, Tingwen | Chen, Xiaoqiang
In this paper, we designed and synthesized three benzothiazole-based fluorescent probes L₁, L₂, and L₃ for zinc ion detection. Among various metal ions, only the zinc ion exhibited fluorescence enhancement at 475 nm accompanied by the blue-shift emission wavelength in HEPES buffer solution containing probes. Through titration experiment, the detection limit of L₁ for zinc ion sensing was calculated to be as low as 7 nM, which showed a high sensitivity. Furthermore, the confocal laser scanning micrographs of HeLa cells demonstrate good cell permeability of probe L₁ and selective detection of zinc ion in living cells. The L₁–Zn²⁺ complex was further used for pyrophosphate (PPi) sensing in HEPES buffer solution, the limit of detection was calculated to be as low as 60 nM. L₁–Zn²⁺ can monitor the enzyme catalyzed degradation process of PPi, thus providing a meaningful way for tracking of zinc ion and pyrophosphate in biological systems.
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