Agrobacterium rhizogenes mediated transformation of the wild soybeans Glycine canescens and G. clandestina: production of transgenic plants of G. canescens
1988
Rech, E.L. | Golds, T.J. | Hammatt, N. | Mulligan, B.J. | Davey, M.R.
Six accessions of Glycine canescens and four of G. clandestina were evaluated for their response to inoculation with Agrobacterium rhizogenes strain LBA 9402. The response was accession-dependent, with 9% to 70% of the seedlings producing roots following infection of the hypocotyl. Excised cotyledons were less responsive than hypocotyls. Roots exhibited plagiotropic and negatively geotropic growth in culture on hormone-free agar medium; those of G. canescens produced shoots on B5 based medium containing 10 mg dm-3 BAP and 0.05 mg dm-3 IBA. Cultured roots and regenerated shoots synthesized opines. DNA sequences homologous to those of pRi TL and TR-DNA were present in a clonally propagated regenerated plant of G. canescens.
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