Effect of superfine grinding on physicochemical properties and endogenous enzyme induced flavonoid transformations of Tartary buckwheat bran
2022
Xiao, Yuan | Zhang, Jian | Zhang, Lianfu
Tartary buckwheat (TB) bran contains endogenous rutin-degrading enzyme (RDE), which can convert flavonoid glycosides into aglycones and ethyl-rutinoside with potential health benefits. However, the large particle size of TB bran limits its application in food industry. The present study aims to combine micronization and endogenous RDE catalysis for the treatment of TB bran. Here, four TB bran samples, with distinct D₅₀ values (the equivalent volume diameters at 50% cumulative volume) of 359, 239, 168 and 42 μm, were prepared by superfine grinding; their physical properties were evaluated. Additionally, with RDE catalysis, the phytochemical compounds, antioxidant and α-glucosidase inhibitory activities of the four bran samples were investigated. Compared to the unprocessed material, TB bran (D₅₀, 42 μm) exhibited the highest amount of aglycones and ethyl-rutinoside after RDE catalysis, with the total content increased from 1 mg/g to 35 mg/g. Importantly, after superfine grinding and RDE catalysis, the antioxidant and α-glucosidase inhibitory activities of TB bran significantly increased. Theoretically, molecular docking verified that the increased aglycone (quercetin), generated after RDE catalysis, is the predominant α-glucosidase inhibitor. Accordingly, the combination of superfine grinding and RDE catalysis is a feasible technology for developing high value-added TB bran products.
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