Effects of high pressure processing on the copigmentation reaction of pelargonidin-3-glucoside and catechin
2019
Zou, Hui | Ma, Yan | Liao, Xiaojun | Wang, Yongtao
The solution absorbance ratios were determined to investigate the effects of high pressure processing on the copigmentation reaction of pelargonidin-3-glucoside and catechin. The theoretical spectra and molecular dynamic simulation were used to calculate the catechin/anthocyanin complex concentration, explore the intrinsic relationship between complex structure and absorbance. Results showed that when interacted with catechin at pH 1.5, the catechin/flavylium had the molar absorption coefficient of 1.78 times at 520 nm. Higher catechin concentration increased the solution absorbance about 1.3 times at pH 3.6 (100, 300, 500 MPa; 60 °C); high pressure processing could significantly accelerate the copigmentation rates at pH 1.5, 25 °C with the ratio of 10:1, and 60 °C with the ratio of 1:1. The solution absorbance at 60 °C was about 1.27 times of the corresponding ones at 20 °C (catechin/anthocyanin ratio of 10:1; 300 and 500 MPa). Higher catechin concentration, pressure and temperature could accelerate the copigmentation reactions.
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