Bovine neutrophil functionality in molybdenum-induced copper deficiency
1998
Cerone, S. | Sansinanea, A. | Streitenberger, S. | Garcia, C. | Auza, N.
The effect of molybdenum - induced copper deficiency on polymorphonuclear leukocytes function was examined. Five female cattle were given molybdenum (30 ppm) and sulphate (225 ppm) to induce experimental secondary copper deficiency. Oxidant production by bovine neutrophils was measured by chemiluminiscence (CL) after stimulation with phorbol myristate acetate (PMA) and opsonized zymosan (OpZ). Lipoperoxidative effects inside of neutrophil, superoxide anion and hydrogen peroxide formation were determined. Copper deficiency was confirmed from decreased serum copper levels and the animals with values less than 5.9 micromole.l-1 were considered deficient. The content of intracellular copper decreased about at 70% in deficient neutrophils compared with the controls. The respiratory burst activity determined by CL was significantly impaired with both stimulants used. Superoxide anion formation was less affected than hydrogen peroxide generation. In addition, we found increased lipid peroxidation. We concluded that the effect of these changes may impair the neutrophil function in the immune system.
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