Optimization of hydrolysis conditions for bovine plasma protein using response surface methodology
2015
Seo, Hyun-Woo | Jung, Eun-Young | Go, Gwang-woong | Kim, Gap-Don | Joo, Seon-Tea | Yang, Han-Sul
The purpose of this study was to establish optimal conditions for the hydrolysis of bovine plasma protein. Response surface methodology was used to model and optimize responses [degree of hydrolysis (DH), 2,2-diphenyl-1-picrydrazyl (DPPH) radical-scavenging activity and Fe2+-chelating activity]. Hydrolysis conditions, such as hydrolysis temperature (46.6–63.4°C), hydrolysis time (98–502min), and hydrolysis pH (6.32–9.68) were selected as the main processing conditions in the hydrolysis of bovine plasma protein. Optimal conditions for maximum DH (%), DPPH radical-scavenging activity (%) and Fe2+-chelating activity (%) of the hydrolyzed bovine plasma protein, were respectively established. We discovered the following three conditions for optimal hydrolysis of bovine plasma: pH of 7.82–8.32, temperature of 54.1°C, and time of 338.4–398.4min. We consequently succeeded in hydrolyzing bovine plasma protein under these conditions and confirmed the various desirable properties of optimal hydrolysis.
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