A fluorescent probe based on reversible Michael addition–elimination reaction for the cycle between cysteine and H₂O₂
2020
Liu, Feiran | Zang, Shunping | Jing, Jing | Zhang, Xiaoling
Cysteine oxidation by H₂O₂, generating either cysteine sulfenic acid (CSOH) or disulfide (CSSC), is involved in redox homeostasis and signaling. Compared with quantification of the cysteine content, monitoring the cysteine dynamics in real-time, in particular, takes on even greater importance. However, existing fluorescent probes suffer from low specificity or irreversible recognition mechanisms. In the present work, we have successfully developed a reversible fluorescent probe for the cycle between cysteine and H₂O₂ based on the Michael addition–elimination reaction. This probe features a specific and quantitative response to cysteine. The reversible detection was realized repeatedly with the addition of cysteine and H₂O₂ in order. We also demonstrated its usage for monitoring exogenous and endogenous cysteine in living cells. Eventually, this probe was capable of imaging cysteine dynamically in real-time.
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