Extraction of soybean isoflavones from soybean meal with aqueous methanol modified supercritical carbon dioxide
2008
Zuo, Y.B. | Zeng, A.W. | Yuan, X.G. | Yu, K.T.
The effects of modifier composition in terms of methanol content in water, modifier concentration, meal particle size and extraction conditions (temperature, pressure and CO₂ flow rate) on isoflavones recovery from soybean meal by supercritical carbon dioxide (SC-CO₂) extraction were investigated. The highest isoflavones recovery attained was 87.3%, at 40°C and 50MPa, using CO₂ flow rate of 9.80kg/h containing a modifier 7.8 mass% of 80% (v/v) aqueous methanol. Using different modifier concentrations (60-100% methanol) at 50MPa and 40°C, it was shown that 80% methanol was optimum for isoflavones extraction. In addition, isoflavones recovery increased with the modifier content in SC-CO₂ up to 10.2%. The optimal particle size was 20-30 mesh with bigger and smaller particles resulting in lower isoflavones recovery. The results showed that increasing the temperature from 40 to 70°C drastically reduced isoflavones recovery at 50MPa. The higher the extraction pressure, the higher was the isoflavones recovery. The extraction rate increased with CO₂ flow rate but the percentage recovery was more or less the same.
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