Thermal gradient deacidification of crude rice bran oil utilizing supercritical carbon dioxide
2001
Dunford, N.T. | King, J.W.
The effect of isothermal and temp. gradient operation of a supercritical fluid fractionation column on the composition of rice bran oil (RBO) fractions was studied. Optimized conditions should allow free fatty acid (FFA) removal, whilst minimizing losses of phytosterols and triacylglycerols (TAG). Application of a temp. gradient along the column was beneficial in reducing the TAG lost in the extract fraction. Utilization of higher temp. in the stripping section improved FFA removal from crude RBO. FFA content of the extract increased, and TAG content decreased with respect to time during the fractionation runs. Increasing the CO2 flow rate from 1.2 to 2 l/min did not affect the extract composition significantly. By using the above approach, it was possible to obtain RBO fractions with similar total sterol ester content to that of the commercially available sterol ester-enriched margarines/spreads. Values were approx. 23 and 21% based on HPLC area measurements for the RBO and margarine/spread, respectively, including fatty acid and ferulic acid sterol esters for the RBO product, but only fatty acid sterol esters for the margarine/spread.
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