pH-dependent aggregation and secretion of soluble monomeric influenza hemagglutinin

Vanlandschoot, P.; Beirnaert, E.; Grooten, J.; Min Jou, W.; Fiers, W.

pH-dependent aggregation and secretion of soluble monomeric influenza hemagglutinin

1998

Vanlandschoot, P. | Beirnaert, E. | Grooten, J. | Min Jou, W. | Fiers, W.

We previously reported the expression of soluble A/Victoria/3/75 (H3N2) hemagglutinin in insect cells and the molecular and immunological structure of an aggregated fraction, only observed in cell supernatant when expression was performed at low pH [23]. Here we report that besides this aggregated a monomeric and possibly a trimeric structure is detected in cell supernatant, irrespective of the pH of the medium. Evidence is presented that the aggregated fraction is generated out of monomeric HA0s molecules due to a low intracellular pH encountered during secretion.

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