Paraburkholderia panacisoli sp. nov., a potentially antagonistic bacterium against the root rot fungal pathogen Cylindrocarpon destructans, isolated from ginseng cultivation soil
2020
Huo, Yue | Chokkalingam, Mohan | Kang, Jong-Pyo | Ahn, Jong-Chan | Yang, Deok-Chun
A new bacterium, designated DCY113ᵀ, was isolated from ginseng cultivation soil in Gochang-gun, South Korea, and its taxonomic position identified by the polyphasic approach. 16S rRNA gene sequence analysis determined that this isolate belongs to the genus Paraburkholderia, and was closest to P. dipogonis DL7ᵀ (98.6%), P. phytofirmans PsJNᵀ (98.5%), P. kirstenboschensis Kb15ᵀ (98.4%) and P. aromaticivorans BNᵀ (98.1%). Strain DCY113ᵀ is Gram-reaction negative, strictly aerobic, rod-shaped, non-motile, and catalase and oxidase positive. The predominant isoprenoid quinone of DCY113ᵀ was ubiquinone Q-8. The major cellular fatty acids were C₁₆:₀, cyclo-C₁₇:₀ and the Summed feature 8 (C₁₈:₁ω7c and/or C₁₈:₁ω6c). The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and an unknown amino lipid (AL1). The G+C content of the genomic DNA was 62.2 mol%. Average nucleotide identity (ANI) between strain DCY113ᵀ and the related Paraburkholderia type strains were below the threshold value for species delineation. This low DNA relatedness in combination with phylogenetic and phenotypic tests indicates that strain DCY113ᵀ cannot be assigned to any recognized species. Strain DCY113ᵀ was also found to have antifungal activity against the pathogenic fungi Cylindrocarpon destructans. In conclusion, this study found DCY113ᵀ to be a novel species within the genus Paraburkholderia, for which the name P. panacisoli is proposed. The type strain is DCY113ᵀ (= KCTC 52951ᵀ = JCM 32098ᵀ).
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