Rapid biochemical synthesis of ¹¹C-labeled single chain variable fragment antibody for immuno-PET by cell-free protein synthesis
2012
Matsuda, Takayoshi | Furumoto, Shozo | Higuchi, Kae | Yokoyama, Jun | Zhang, Ming-Rong | Yanai, Kazuhiko | Iwata, Ren | Kigawa, Takanori
Immuno-PET is a promising approach for improved cancer diagnosis, by taking advantage of the high specificity of antibodies. Here, we present a novel cell-free protein synthesis method for preparing a positron emitter labeled-antibody. Functional anti-human EGFRvIII single chain Fv, MR1-1, was successfully labeled with carbon-11 (half-life=20.4min) in 5min (36% yield) by the direct incorporation of the clinical PET tracer, l-[¹¹C]methionine. The product [¹¹C]MR1-1 was easily and rapidly isolated with high radiochemical purity (>95%) from the reaction solution, by affinity purification. This method would be widely applicable to the preparation of radiolabeled antibodies for PET imaging.
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