Évaluation de l’activité antioxydante de Berberis vulgaris L | Evaluation of the antioxidant activity of Berberis vulgaris L
2014
Mezouar, D. | Lahfa, F.B. | Djaziri, R. | Boucherit-Otmani, Z.
Natural substances from plants have multiple interests exploited in biotechnology industry both in food, cosmetic and pharmaceutical. These compounds include a large proportion of secondary metabolites that are illustrated in many areas and even in therapy. In this context, we investigated the antioxidant activity of Berberis vulgaris, a plant used in Algeria, and it is particularly used in the Tlemcen region. After preparation of extracts from the root bark of Berberis vulgaris, total alkaloids were analyzed by thin layer chromatography, indicating the presence of six tasks. A quantitative analysis of polyphenols and flavonoids in methanolic extract was carried out, showing a content of 10.48 mg GAE/g of total polyphenols and 2.05 mg CEQ/g of flavonoids. The antioxidant activity of hydromethanolic extracts under reflux and total alkaloids was evaluated using two methods: reducing power and DPPH free radical scavenging. The results showed a high activity of methanolic extract than the total alkaloids, with an IC₅₀ of 1.40 mg/ml.
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