Excised twig assay to evaluate cherry rootstocks for tolerance to Pseudomonas syringae pv. syringae
1992
Krzesinska, E.Z. | Azarenko, A.N.M.
An excised twig assay was developed to evaluate cherry rootstocks (hybrids of Prunus avium L.; P. canescens Bois.; P. cerasus L.; P. fruticosa Pall.; P. mahaleb L.; P. pseudocerasus Lindl.) for their tolerance to Pseudomonas syringae pv. syringae van Hall. Twigs of 'Napoleon', 'Corum', and F12/1 in 1988 and 10 more rootstocks in 1989 were inoculated with water, one avirulent (K4), or one of three virulent strains (W4N54, AP1, and B-15) of bacteria at 10(5), 10(6), and 10(7) colony forming units (cfu)/ml in 1988 and with 10(7) cfu/ml in 1989. Evaluation of browning and gummosis at the inoculation site after incubation for 4 weeks at 15 C and high relative humidity revealed no gummosis or browning on twigs inoculated with water or the avirulent strain. The amount of browning and gummosis induced by concentrations of 10(6) and 10(7) cfu/ml of the virulent strains was, in general, not different within genotypes. 'Napoleon' and 'Corum' had significantly higher browning and gummosis ratings in 1989 than F12/1 and the 10 rootstock selections, most of which did not differ from F12/1. Rootstocks Gisela (Gi.) 172-9 and Gi. 169-15 had higher incision browning than F12/1 in some instances.
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