A rapid self-assembled hybrid bio-microflowers of alpha–amylase with enhanced activity
2020
Maurya, Shefali S. | Nadar, Shamraja S. | Rathod, Virendra K.
In conventional preparation of enzyme embedded organic-inorganic hybrid flower-like nanostructures, usually it requires three days which is time-consuming and limits their widespread applications. In this context, alpha–amylase hybrid bio-microflowers were prepared by simple, efficient and rapid method in the presence of chitosan via inotropic gelation and biomineralization approach. The hybrid bio-microflowers was synthesized within 6 h with ∼140 % enhanced catalytic activity. The prepared hybrid bio-microflowers were characterized by FT–IR, SEM, and PXRD. The hybrid bio-microflowers exhibited higher rate of reaction (Vₘₐₓ) and outstanding thermo-stability (in the temperature range 55–75 °C). Further, hybrid bio-microflowers showed magnificent reusability (upto eight cycles) and long-term storage stability (for about 30 days). In the end, in starch hydrolytic study, immobilized alpha–amylase exhibited higher hydrolytic potential towards corn, wheat and potato starch as compared to free form. This designed hybrid bio-microflowers can be employed as an engineered biocatalyst in industrial applications.
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