Sequence diversity and in silico structure prediction of the vacuolar invertase inhibitor gene from potato (Solanum tuberosum L.) cultivars differing in sugar content
2019
Datir, Sagar S | Mirikar, Duhita | RaviKumar, Ameeta
The aim of this study was to examine the variations in sugar content and identify the polymorphism in vacuolar invertase inhibitor (INH2) gene from Indian non-processing (Kufri Jyoti, Kufri Pukhraj and PU1) and exotic processing (Atlantic and Frito Lay-1533) potato cultivars. Upon cold storage (4°C) processing cultivars maintained low reducing sugars as compared to non-processing cultivars. Sequencing of the INH2 gene identified four alleles of which three identified as novel alleles. A total twelve SNPs resulted in silent mutations, with five conferring the amino acid substitutions. Phylogenetic analysis suggests a highly conserved nature of the INH2 gene. The 3D predicted structures generated for all the alleles revealed slight variations in the orientation of the helices (α1-3) in N-terminal region. Sequence polymorphism observed in INH2 alleles in processing and non-processing potato cultivars can be correlated with the observed variations in the sugar content suggesting a possible role in cold-induced sweetening.
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