Disentangling a shared trait: male control over mate guarding duration revealed by a mate exchange experiment

Shared behavioural traits result from the interaction of two or more individuals, making it difficult to discern which individual is in control of the behaviour of interest. Especially in the case of shared reproductive traits such as mating duration or mate guarding duration is this an important issue to resolve, because these are potentially closely connected to fitness and are likely to exhibit sexual conflict. Here, we sought to disentangle which sex controls mate guarding duration in the tropical house cricket Gryllodes sigillatus, a species in which mate guarding and nuptial feeding by the male have been proposed to prevent premature removal of the transferred spermatophore by the female. To do so, we performed a series of mating experiments in a paired design, in which the first mating dyad was allowed to start mating some time before the second dyad. Once both dyads were in the mate guarding phase, we then interrupted them and exchanged partners, enabling us to determine whether the remaining guarding duration depended more on the duration of guarding already performed by the male in the new dyad (implying male control) or on the guarding already received by the female (implying female control). We found that the time a female was guarded overall was significantly affected by how long the exchanged male had already engaged in mate guarding with the previous female, but conversely, the total time males guarded both females was unaffected by the duration of guarding that the exchanged female had previously received. Our data thus clearly demonstrate that males rather than females control mate guarding duration and adjust the duration according to females’ weight. SIGNIFICANCE STATEMENT: It is not easy to determine which individual is in control of a shared behavioural trait (SBT). This information could provide insight into selection pressure on one sex and could help us understand differences in SBTs between related species or between different dyads of one species. We used a relative novel but simple method to disentangle a SBT in a cricket. We performed mating experiments and exchanged the mating partners after copulation within the mate guarding phase and measured the total guarding duration. Our analyses showed males were not influenced by the exchange and guarded as long as expected regardless how long the female were guarded before by another male. Our data suggest males are likely in control of mate guarding duration, and they have no ability to recognize post-copulatory mate exchange.