Carnauba wax p-methoxycinnamic diesters: Characterisation, antioxidant activity and simulated gastrointestinal digestion followed by in vitro bioaccessibility
2016
Freitas, Claisa Andréa Silva | Vieira, Ícaro Gusmão Pinto | Sousa, Paulo Henrique Machado | Muniz, Celli Rodrigues | Gonzaga, Maria Leônia da Costa | Guedes, Maria Izabel Florindo
The beneficial biological effects of cinnamic acid derivatives and the lack of studies on the antioxidant activity and bioavailability of cinnamic esters from carnauba wax, diesters were extracted from carnauba wax powder. Their structural, physical and morphological characteristics, antioxidant activity and in vitro bioaccessibility were measured. p-Methoxycinnamic diester (PCO-C) was identified, which has a crystalline, apolar structure and exhibited significant antioxidant activity (107.27±3.92μM Trolox/g of dry weight) before and after simulated in vitro gastrointestinal digestion and 32.46% bioaccessibility. In human cells, PCO-C (250μg/mL) inhibited the production of intracellular reactive oxygen species, with an effect similar to that of Trolox (80μM). Thermogravimetric analysis showed that PCO-C had high thermal stability and high UV absorption between 250 and 350nm. These results indicate that this compound is promising as an antioxidant for pharmaceutical and food industry applications, such as the development of active packaging and functional foods.
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