Microsatellite loci for the southern pine beetle (Dendroctonus frontalis) and cross-species amplification in Dendroctonus
2007
SCHREY, NATALIE M. | SCHREY, AARON W. | HEIST, EDWARD J. | REEVE, JOHN D.
We developed microsatellite loci for the southern pine beetle (Dendroctonus frontalis). Twelve microsatellite loci were identified. Eight loci were polymorphic and sufficiently variable in 62 individuals (expected heterozygosity ranged from 0.707 to 0.880) to investigate population structure. All loci conformed to HWE except Dfr-14, which showed heterozygote excess, and no two loci deviated from linkage equilibrium. The loci were tested for cross-species amplification in four species of Dendroctonus (D. valens, D. terebrans, D. brevicomis, and D. ponderosae). Seven loci were polymorphic in at least one of the species tested.
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