Studies of the determination of the bacterium coli type i content of farm water supplies
1955
THOMAS, S.B. | CLEGG, L.F.L. | CUTHBERT, W.A. | OXLEY, C.D. | SCARLETT, C.A. | WESTWATER, C.H. | JONES, G ELIS | McCLEMONT, J. | MORRIS, C.S. | NICHOLS, AGNES A.
SUMMARY: A - comparison of the suitability of brilliant green bile broth and MacConkey's broth at 44° for the detection of Bacterium coli type I in farm water supplies, showed that 83.1% of the samples had no difference in the number of positive tubes at 44°, and only 5 samples (1.7%) had a significantly higher number of positive tubes in MacConkey's broth. Of 707 strains of coli-aerogenes bacteria isolated from 44° positive tubes of both media, 94.5% were Bact. coli type I. Strains of Bact. coli type II and Bact. aerogenes type I which were 44° positive constituted 3.7% and 0.4% respectively, all of which were indole negative at 44°. In addition there were 10 strains (1.4%) of 44° positive Intermediate type II, 9 of which were indole positive at 44°. An appreciable number (6.6%) of Bact. coli type I strains failed to give a positive indole reaction in 24 hr at 44°.
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