Sensitivity of photoelctrocehmical aptasensor using spiral nanorods for detecting antiobiotic levels in experimental and real samples
2022
Sun, Yimeng | Ma, Cong | Wu, Man | Jia, Chunping | Feng, Shilun | Zhao, Jianlong | Liang, Lijuan
Given increasing concern regarding antibiotic environmental contamination, there is immediate need to monitor antibiotic levels to effectively control pollution. In this study, we used a photoelectrochemical aptasensor based on TiO₂@MoS₂ spiral nanoarrays to detect chloramphenicol (CAP) in antibiotics. Nanoarrays were directly grown on fluorine-doped tin oxide (FTO) conductive glass with excellent biochemical stability, while aptamer-SH were immobilized by chemical binding on a synthetic TiO₂@MoS₂ nanoarray. Results show that the photocurrents were reduced in the presence of photoelectrochemistry associated with specific selection of aptamer for CAP. When the measurement of the fabricated nanomaterial chip was carried out using a three-electrode system, we found a highly specific and stable detection of chloramphenicol that ranged between 0.1 pM and 1 μM, with the detection limit of 0.1 pM. In addition, we obtained satisfactory results when real sample were used to validate the potential of photoelectrochemical (PEC) aptasensor for detecting chloramphenicol content in milk. Our results demonstrate that photoelectrochemical aptasensor is conducive to the development of less toxic multifunctional nanomaterials, making the biosensor more robust and environmentally friendly. Therefore, photoelectrochemical aptasensor can be widely applied in the field of environmental monitoring.
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