Incorporation of T4 Phage DNA into a Specific DNA Fraction from the Higher Plant Matthiola incana
2014
Gradmann-Rebel, Waltraud | Hemleben, Vera
Isolated T₄ phage DNA (ϱ = 1.694 g/ml) is applied to seedlings of the crucifer Maltliiola incana (DNA density ϱ = 1.698 g/ml). The phage DNA can partly be reextracted from the plants in a specific DNA fraction, which is predominantly characterized by its unusual high density (high density complex = HDC; ϱ = 1.724 g/ml). DNA: DNA hybridization studies show that phage specific DNA sequences are preserved in the HDC. Results of BrdUrd labeling of the plant DNA before and during incubation with T₄ DNA suggest that the HDC is composed of T₄ DNA and a plant DNA component of high density. The analysis of ultrasonicated HDC confirms this suggestion. The ability of plant cells to recognize and handle T₄ DNA specifically is discussed.
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