Natural heterogeneity of chymosin and pepsin in extracts of bovine stomachs
2005
Rampilli, M. | Larsen, R. | Harboe, M.
The two main components, chymosin and pepsin, in calf and adult bovine rennet show heterogeneity with respect to their charge and activity. A large number of single stomachs have been extracted and their isoenzymes have been analysed by HPLC. The results showed three genetic forms of chymosin A, B and C and these are present in individual stomachs in the following combinations: AA, BB, CC, AB, AC and BC. The C variant, which can be distinguished from the degradation product of chymosin A, has never been characterised before. All three chymosins have been purified and partially characterised. Also bovine pepsins show a natural variability: the ratio between the main pepsin fractions varies according to the age of the animal and it therefore provides a criterion to distinguish between pepsin from calf and adult bovine animals.
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